Thank you for the post @Srikanth-R
Regardless of platform (HW Series or VM) and regardless of PAN-OS version, the reboot is required for enabling/disabling jumbo Frames to take an effect: https://docs.paloaltonetworks.com/vm-series/10-1/vm-series-deployment/about-the-vm-series-firewall/e...
I would recommend you to schedule a maintenance window to proceed with this change.
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