If you've implemented DNS sinkhole Anti-Spyware protection in your firewall, hosts attempting to hit the sinkhole IP would be those trying to communicate through command-and-control, so it can help pinpoint the infections. You can also look for entries in the Threat logs for Anti-spyware signatures (that are not DNS based) to see if you can spot any malicious activity.
If you have Traps and Magnifier, you may also be able to detect behavioral anomalies which can help pinpoint the infected hosts.
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